Illumina NovaSeq6000 Sequencing of Dock8_017 Metatranscriptomic Paire... - SRA

SRX21299275: Illumina NovaSeq6000 Sequencing of Dock8_017 Metatranscriptomic Paired-end Library
2 ILLUMINA (Illumina NovaSeq 6000) runs: 17.2M spots, 5.2G bases, 1.9Gb downloads

Design: Illumina NovaSeq6000 2x151 sequencing of Dock8_017 metatranscriptomic paired-end dual index library

Submitted by: NIH Intramural Sequencing Center (NISC)

Study: The skin microbiome of DOCK8-deficient patients

PRJNA471898 • SRP157584 • All experiments • All runs

show Abstracthide Abstract

Dedicator of cytokinesis 8 (DOCK8) deficiency is a rare autosomal recessive, combined immunodeficiency that features cutaneous viral-associated manifestations, including molluscum contagiosum, herpesvirus infections, and warts (HPV infections) along with atopic dermatitis. We present a comprehensive investigation of the skin microbiome of DOCK8-deficient patients, focusing on the skin virome. We utilized both reference-based and reference-free analyses to investigate the microbial (viral) 'dark matter' and discovered a large number of novel human papillomavirus genomes.

Sample: RNAseq library

SAMN36811623 • SRS18548208 • All experiments • All runs

Organism: skin metagenome

Library:

Name: BCC3572_1_L19316510

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: METATRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Libraries were prepared using the stranded total RNA prep kit (Illumina, 20040529) with AMPure XP beads (Agencourt, A63881) following an initial removal of rRNA with the FastSelect -rRNA H/M/R kit (Qiagen, 334386). Unique-dual indexes (Illumina, 20627581) were ligated to the cDNA libraries. Library quality was assessed using Qubit and Bioanalyzer.

Spot descriptor:

1 forward152 reverse

Pipeline: show...hide...

Step	Prev Step	Program	Version
1	NIL	RTA	3.4.4

Runs: 2 runs, 17.2M spots, 5.2G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR25570600	9,194,756	2.8G	1,016Mb	2023-08-15
SRR25570601	8,019,320	2.4G	900.6Mb	2023-08-15

ID:: 28736235

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